全文获取类型
收费全文 | 37877篇 |
免费 | 3154篇 |
国内免费 | 2753篇 |
出版年
2024年 | 14篇 |
2023年 | 437篇 |
2022年 | 523篇 |
2021年 | 1929篇 |
2020年 | 1355篇 |
2019年 | 1711篇 |
2018年 | 1722篇 |
2017年 | 1174篇 |
2016年 | 1642篇 |
2015年 | 2401篇 |
2014年 | 2827篇 |
2013年 | 3074篇 |
2012年 | 3575篇 |
2011年 | 3165篇 |
2010年 | 1987篇 |
2009年 | 1614篇 |
2008年 | 1968篇 |
2007年 | 1718篇 |
2006年 | 1586篇 |
2005年 | 1285篇 |
2004年 | 1052篇 |
2003年 | 909篇 |
2002年 | 758篇 |
2001年 | 664篇 |
2000年 | 587篇 |
1999年 | 622篇 |
1998年 | 343篇 |
1997年 | 357篇 |
1996年 | 336篇 |
1995年 | 317篇 |
1994年 | 332篇 |
1993年 | 263篇 |
1992年 | 311篇 |
1991年 | 241篇 |
1990年 | 213篇 |
1989年 | 189篇 |
1988年 | 127篇 |
1987年 | 101篇 |
1986年 | 92篇 |
1985年 | 86篇 |
1984年 | 59篇 |
1983年 | 53篇 |
1982年 | 34篇 |
1981年 | 9篇 |
1980年 | 9篇 |
1979年 | 11篇 |
1976年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
Mary T. Doan Michael D. Neinast Erika L. Varner Kenneth C. Bedi David Bartee Helen Jiang Sophie Trefely Peining Xu Jay P. Singh Cholsoon Jang J. Eduardo Rame Donita C. Brady Jordan L. Meier Kenneth B. Marguiles Zoltan Arany Nathaniel W. Snyder 《Journal of lipid research》2022,63(6):100224
Anabolic metabolism of carbon in mammals is mediated via the one- and two-carbon carriers S-adenosyl methionine and acetyl-coenzyme A. In contrast, anabolic metabolism of three-carbon units via propionate has not been shown to extensively occur. Mammals are primarily thought to oxidize the three-carbon short chain fatty acid propionate by shunting propionyl-CoA to succinyl-CoA for entry into the TCA cycle. Here, we found that this may not be absolute as, in mammals, one nonoxidative fate of propionyl-CoA is to condense to two three-carbon units into a six-carbon trans-2-methyl-2-pentenoyl-CoA (2M2PE-CoA). We confirmed this reaction pathway using purified protein extracts provided limited substrates and verified the product via LC-MS using a synthetic standard. In whole-body in vivo stable isotope tracing following infusion of 13C-labeled valine at steady state, 2M2PE-CoA was found to form via propionyl-CoA in multiple murine tissues, including heart, kidney, and to a lesser degree, in brown adipose tissue, liver, and tibialis anterior muscle. Using ex vivo isotope tracing, we found that 2M2PE-CoA also formed in human myocardial tissue incubated with propionate to a limited extent. While the complete enzymology of this pathway remains to be elucidated, these results confirm the in vivo existence of at least one anabolic three- to six-carbon reaction conserved in humans and mice that utilizes propionate. 相似文献
103.
104.
Evidence shows that an abnormal deposition of amyloid beta-peptide25–35 (Aβ25–35) was the primary cause of the pathogenesis of Alzheimer’s disease (AD). And the elimination of Aβ25–35 is considered an important target for the treatment of AD. Triptolide (TP), isolated from Tripterygium wilfordii Hook.f. (TWHF), has been shown to possess a broad spectrum of biological profiles, including neurotrophic and neuroprotective effects. In our study investigating the effect and potential mechanism of triptolide on cytotoxicity of differentiated rat pheochromocytoma cell line (the PC12 cell line is often used as a neuronal developmental model) induced by Amyloid-Beta25–35 (Aβ25–35), we used 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay, flow cytometry, Western blot, and acridine orange staining to detect whether triptolide could inhibit Aβ25–35–induced cell apoptosis. We focused on the potential role of the autophagy pathway in Aβ25–35-treated differentiated PC12 cells. Our experiments show that cell viability is significantly decreased, and the apoptosis increased in Aβ25–35-treated differentiated PC12 cells. Meanwhile, Aβ25–35 treatment increased the expression of microtubule-associated protein light chain 3 II (LC3 II), which indicates an activation of autophagy. However, triptolide could protect differentiated PC12 cells against Aβ25–35-induced cytotoxicity and attenuate Aβ25–35-induced differentiated PC12 cell apoptosis. Triptolide could also suppress the level of autophagy. In order to assess the effect of autophagy on the protective effects of triptolide in differentiated PC12 cells treated with Aβ25–35, we used 3-Methyladenine (3-MA, an autophagy inhibitor) and rapamycin (an autophagy activator). MTT assay showed that 3-MA elevated cell viability compared with the Aβ25–35-treated group and rapamycin inhibits the protection of triptolide. These results suggest that triptolide will repair the neurological damage in AD caused by deposition of Aβ25–35 via the autophagy pathway, all of which may provide an exciting view of the potential application of triptolide or TWHF as a future research for AD. 相似文献
105.
106.
帕金森病(Parkinson disease,PD)是一种复杂的中枢神经系统退行性疾病,主要病理特征为黑质致密部多巴胺神经元的进行性丧失.目前PD主要治疗手段包括药物和手术.但药物存在神经保护活性不足、缺乏对因治疗、晚期无药可用等问题,手术治疗风险较大.近年来,细胞重编程技术取得突破性进展,由重编程产生的诱导多能干细胞(induced pluripotent stem cells,iPSCs)、诱导多巴胺神经元(induced dopamine neurons,iDNs)和诱导神经干细胞(induced neural stem cells,i NSCs)可用于治疗PD.移植iPSCs分化而来的多巴胺能神经元、iDNs和iNSCs至相应脑区,可起到神经替代与修复作用,有效治疗PD.本文重点介绍细胞重编程的机制,总结iPSCs、iDNs和iNSCs治疗PD的优缺点,并阐述尚存在的挑战,探讨可能的解决方案. 相似文献
107.
108.
109.
110.